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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1976 Sep;73(9):3156–3159. doi: 10.1073/pnas.73.9.3156

DNA dependent synthesis of protein L12 from escherichia coli ribosomes, in vitro.

F Chu, H F Kung, P Caldwell, H Weissbach, N Brot
PMCID: PMC430964  PMID: 787986

Abstract

The in vitro synthesis of ribosomal protein L12 has been obtained in a coupled system with DNA extracted from the transducing phage lambdarifd18 as template. In addition, a second protein (molecular weight of 16,000) with immunological, chemical, and ribosome-binding characteristics similar to L12 is formed in this in vitro system. The synthesis of both proteins is depressed by one-half when guanosine-5'-diphosphate-3'-diphosphate is added to the reaction mixture.

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Selected References

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