TABLE 2.
Overview of phylotypes from within Guaymas Basin, and outside Guaymas Basin, with highest BLAST sequence similarity to ones detected at Everest Mounda
| OTU name | Closest BLAST hit from Guaymas Basin (accession no.) | Habitat | % similarity | Reference | Closest BLAST hit outside Guaymas Basin (accession no.) | Origin | % similarity | Reference(s) |
|---|---|---|---|---|---|---|---|---|
| mcrA genes | ||||||||
| GB-EvMd-Mhalo-mcrA | Guaymas_37enr_mcrA 28 (FR682810) | Hydrothermal sediment | 99 | 11 | Methanohalophilus halophilus (AB703633) | Shark Bay hypersaline microbial mat | 86 | 21 |
| GB-EvMd-GrpE-mcrA | 4559–4-2-MCR1 (JX826408) | Hydrothermal vent | 98 | Y. He and F. Wang, unpublished | 25H-0A-16 (FJ403608) | Sea of Ohkotsk cold seeps | 99 | 22 |
| GB-EvMd-ANME-2-mcrA-2 | 4559-3-R1-MCR65 (JX826416) | Hydrothermal vent | 93 | Y. He and F. Wang, unpublished | C09b (HQ454478) | Napoli mud volcano | 97 | 23 |
| GB-EvMd-ANME-2-mcrA-1 | 4486_MCRird_191 (JF937817) | Hydrothermal sediment | 99 | 12 | 25H-200A-3 (FJ403654) | Sea of Ohkotsk cold seeps | 99 | Zhang et al., unpublished |
| GB-EvMd-ANME-2-mcrA-3 | Guaymas_37enr_mcrA 92 (FR682811) | Hydrothermal sediment | 93 | 11 | p760_M_1.03 (AB305373) | Okinawa Trough hydrothermal sediment | 95 | 24 |
| GB-EvMd-Methermicoc-mcrA-2 | A07 (AY837773) | Hydrothermal sediment | 82 | 10 | PS23SGXP401 (GU357470) | Hot Alaskan oil field | 96 | 25 |
| GB-EvMd-Methermicoc-mcrA-1 | A07 (AY837773) | Hydrothermal sediment | 85 | 10 | FS448_53 (HQ635543) | Mariana Arc, hydrothermal vent fluid | 94 | 26, 27 |
| GB-EvMd-Methermicoc-mcrA-3 | A07 (AY837773) | Hydrothermal sediment | 77 | 10 | PS23SGXP401 (GU357470) | Hot Alaskan oil field | 83 | 25 |
| GB-EvMd-Mmseep-mcrA-2 | 4559-3-R1-MCR12 (JX826415) | Hydrothermal vent | 82 | Y. He and F. Wang, unpublished | 40H-260A-4 (FJ403643) | Sea of Ohkotsk cold seep | 98 | Zhang et al., unpublished |
| GB-EvMd-Mmseep-mcrA-1 | None | 25H-270A-7 (FJ403632) | Sea of Ohkotsk cold seep | 96 | Zhang et al., unpublished | |||
| GB-EvMd-Mplanus-mcrA | 4559-3-R1-MCR12 (JX826415) | Hydrothermal vent | 99 | Y. He and F. Wang, unpublished | LW-6 (JX430031) | Min River estuary | 92 | C.-X. She and C. Tong, unpublished |
| GB-EvMd-Mcull-mcrA | C01 (AY837767) | Hydrothermal sediment | 99 | 10 | ATB-EN-10393-M138 (FJ226731) | Agricultural biogas plant | 87 | 28 |
| GB-EvMd-Mcoc-mcrA-1 | Methanocaldococcus jannaschii (L77117) | Hydrothermal sediment | 99 | 19 | FS625_56 (HQ635721) | Juan de Fuca Ridge hydrothermal vent fluid | 99 | 26 |
| GB-EvMd-Mcoc-mcrA-2 | 4559-3-R1-MCR2 (JX826413) | Hydrothermal sediment | 96 | Y. He and F. Wang, unpublished | A4Sx12_84 (HQ635491) | Juan de Fuca Ridge hydrothermal vent fluid | 95 | 26 |
| GB-EvMd-ANME-1-mcrA-1 | None | NapMat-8_10-mcrE03 (HM004884) | Napoli mud volcano hypersaline sediment | 92 | 23 | |||
| GB-EvMd-ANME-1-mcrA-2 | Guaymas_50enr_mcrA 63 (FR682818) | Hydrothermal sediment | 98 | 11 | NapK-40_60-mcrH12 (HM005070) | Napoli mud volcano hypersaline sediment | 92 | 29 |
| GB-EvMd-DbrGrpII-mcrA-1 | 4483_MCRird_285 (JF937797) | Hydrothermal sediment | 72 | 12 | M_mcrA-11 (JQ406852) | Huabei oil field production water | 72 | 30 |
| GB-EvMd-DbrGrpII-mcrA-2 | 4483_MCRird_285 (JF937797) | Hydrothermal sediment | 71 | 12 | M_mcrA-11 (JQ406852) | Huabei oil field production water | 72 | 30 |
| GB-EvMd-DbrGrpIII-mcrA-1 | Methanopyrus kandleri | Hydrothermal sediment | 71 | 20 | FS625_63 (HQ635729) | Juan de Fuca Ridge hydrothermal vent fluid | 74 | 26 |
| GB-EvMd-DbrGrpIII-mcrA-2 | Methanopyrus kandleri | Hydrothermal sediment | 71 | 20 | FS625_63 (HQ635729) | Juan de Fuca Ridge hydrothermal vent fluid | 75 | 26 |
| GB-EvMd-MpyralesGrpI-mcrA | Methanopyrus kandleri | Hydrothermal sediment | 88 | 20 | MCR-A (AY354023) | EPR diffuse vent covered by mats | 94 | 31 |
| GB-EvMd-MpyralesGrpII-mcrA-1 | Methanopyrus kandleri | Hydrothermal sediment | 86 | 20 | MCR-B (AY354018) | EPR diffuse vent covered by mats | 89 | 31 |
| GB-EvMd-MpyralesGrpII-mcrA-2 | Methanopyrus kandleri | Hydrothermal sediment | 86 | 20 | MCR-B (AY354018) | EPR diffuse vent covered by mats | 89 | 31 |
| GB-EvMd-Mpyrus-mcrA-2 | Methanopyrus kandleri | Hydrothermal sediment | 99 | 20 | 1crD2T36 (FN650318) | Rainbow ultramafic hydrothermal vent fluid | 89 | 32 |
| GB-EvMd-Mpyrus-mcrA-1 | Methanopyrus kandleri | Hydrothermal sediment | 99 | 20 | 1crD2T36 (FN650318) | Rainbow ultramafic hydrothermal vent fluid | 90 | 32 |
| Archaeal 16S rRNA genes | ||||||||
| GB-EvMd-Methermicoc-A | 4A08 (AY835419) | Hydrothermal sediment | 97 | 10 | Methermicoccus shengliensis (NR_043960) | Shengli oil field, production water | 99 | 44 |
| GB-EvMd-Methermicoc-C | 4A08 (AY835419) | Hydrothermal sediment | 100 | 10 | Methermicoccus shengliensis (NR_043960) | Shengli oil field, production water | 99 | 44 |
| GB-EvMd-Halo-C | GUAY_37enr_Arch72 (FR682485) | Hydrothermal sediment | 99 | 51 | SS_WC_06 (FJ656258) | Salton Sea, hypersaline lake sediment | 99 | 56, 57 |
| GB-EvMd-Mcull-A | 7H12 (AY835412) | Hydrothermal sediment | 99 | 10 | QHYA-25 (JF741950) | Oil reservoir | 96 | Tang et al., unpublished |
| GB-EvMd-Mcull-B | 7H12 (AY835412) | Hydrothermal sediment | 100 | 10 | Methanoculleus sp. clone A3 (AJ133793) | Freshwater sediment enriched with hexadecane | 96 | 58 |
| GB-EvMd-MBGD-A | 4559-4-C2-82 (JX507259) | Hydrothermal vent | 98 | Y. He and F. Wang, unpublished | KSTwh-C1-7-A-028 (JQ611039) | Kueishan Is shallow hydrothermal field | 90 | Lin et al., unpublished |
| GB-EvMd-MBGD-B | 4559-4-C2-82 (JX507259) | Hydrothermal vent | 98 | Y. He and F. Wang, unpublished | IAN1-62 (AB175594) | Oknawa Trough, hydrothermal vent chimney | 92 | 59 |
| GB-EvMd-Mplasmatales | 7C08 (AY835423) | Hydrothermal sediment | 99 | 10 | 0DA-79 (JQ772440) | High-temperature oil reservoir | 99 | Z. Fang, unpublished |
| GB-EvMd-DHVE5-A | None | a87R29 (DQ417481) | EPR, seafloor basaltic flanks | 97 | 60 | |||
| GB-EvMd-DHVE5-B | None | ARCHDER07_1A1 (FN598017) | Almeria, Spain, seawater-processed activated sludge | 88 | 61 | |||
| GB-EvMd-ANME-1a-B | GUAY_50enr_Arch41 (FR682490) | Hydrothermal sediment | 99 | 11 | MC118_26C2 (HM600925) | Gulf of Mexico hydrocarbon seep sediment | 99 | Twing et al., unpublished |
| GB-EvMd-ANME-1a-C | Arch4483_112 (JF937751) | Hydrothermal sediment | 99 | 12 | MC118_26C2 (HM600925) | Gulf of Mexico hydrocarbon seep sediment | 99 | Twing et al., unpublished |
| GB-EvMd-ANME-1b-A | BG410(1)9 (JQ740749) | Hydrothermal vent fluid | 99 | 27 | FS725(3)73 (JQ740760) | Endeavor Segment hydrothermal fluid | 97 | 27 |
| GB-EvMd-ANME-1b-C | GBa2r032 (AF419632) | Hydrothermal sediment | 99 | 4 | FS625(3)16 (JQ740759) | Endeavor Segment hydrothermal fluid | 99 | 27 |
| GB-EvMd-GBEury-G | Arch4486_075 (JF937738) | Hydrothermal sediment | 99 | 12 | KZNMV-25-A37 (FJ712390) | Kazan Mud Volcano, hydrate sediment | 100 | 62 |
| GB-EvMd-GBEury-E | Arch4486_075 (JF937738) | Hydrothermal sediment | 96 | 12 | Arch-Q12 (JQ241421) | Huabei oil field | 98 | 63 |
| GB-EvMd-GBEury-K | 4E09 (AY835427) | Hydrothermal sediment | 98 | 10 | KZNMV-25-A37 (FJ712390) | Kazan Mud Volcano, hydrate sediment | 95 | 62 |
| GB-EvMd-ThCoc-D | Thermococcus sp. GB18 (FJ862790) | Hydrothermal sediment | 99 | 52 | Thermococcus alcaliphilus (NR040870) | Vulcano submarine solfataric field | 99 | 64 |
| GB-EvMd-ThCoc-E | T. mexicalis (AY099181) | Hydrothermal sediment | 99 | 53 | HibPWCl-ARC3 (JF789485) | Hibernia oil field, production water | 99 | Yeung et al., unpublished |
| GB-EvMd-ThCoc-F | T.coccus guaymasensis (JQ346762) | Hydrothermal sediment | 98 | 53 | Thermococcus sp. Ax00-27 (AY559130) | Axial seamount, diffuse hydrothermal vent | 97 | J. A. Huber and J. A. Baross, unpublished |
| GB-EvMd-ThCoc-G | G26_C48 (AF356631) | Sulfide chimney | 95 | K. Longnecker and A.-L. Reysenbach, unpublished | Q2-a15.seq (FR852947) | Hydrothermal vent chimney | 94 | J. Li, unpublished |
| GB-EvMd-Archaeoglobales | Archaeoglobus profundus (AF297529) | Hydrothermal sediment | 93 | 54 | PW15.7A (EU573156) | North Sea chalk petroleum reservoir | 98 | 65 |
| GB-EvMd-DHVEG | 90-PY-10 (FR692155) | Hydrothermal sediment | 99 | 55 | pCIRA-L (AB095122) | Central Indian Ridge hydrothermal field | 96 | 66 |
| GB-EvMd-AcidProf-A | G26_C56 (AF356635) | Sulfide chimney | 98 | K. Longnecker and A.-L. Reysenbach, unpublished | HTM1039Pn-A31 (AB611429) | Okinawa Trough, polychaete nest, hydroth. vent | 99 | 67 |
| GB-EvMd-AcidProf-B | G26_C56 (AF356635) | Sulfide chimney | 98 | K. Longnecker and A.-L. Reysenbach, unpublished | Aciduliprofundum isolate EPR07-39 (FR865186) | EPR hydrothermal vent | 99 | 68 |
| GB-EvMd-AcidProf-F | G26_C56 (AF356635) | Sulfide chimney | 95 | K. Longnecker and A.-L. Reysenbach, unpublished | Aciduliprofundum isolate EPR07-39 (FR865186) | EPR hydrothermal vent | 96 | 68 |
| GB-EvMd-MCG-A | 4559-4-C2-11 (JX507250) | Hydrothermal vent | 99 | Y. He and F. Wang, unpublished | PNG_Kap4_A49 (JF935159) | Alkaline hot spring | 99 | Amend et al., unpublished |
| GB-EvMd-MCG-B | 4559-4-C2-9-5 (JX507244) | Hydrothermal vent | 97 | Y. He and F. Wang, unpublished | A257-49 (FN554070) | Logatchev ultramafic hydrothermal vent field | 98 | 69 |
| GB-EvMd-Thermoproteales-1 | 4559-4-C2-61 (JX507245) | Hydrothermal vent | 100 | Y. He and F. Wang, unpublished | HTM1036Pn-A14 (AB611436) | Okinawa Trough, polychaete nest, hydrothermal vent | 96 | 67 |
a
Boldface indicates the environmental sequences with the highest sequence similarity to the operational taxonomic units (OTU) detected in this study. Unpublished data: Amend et al., J. P. Amend, L. C. Burcea, and D. R. Meyer-Dombard; Lin et al., L.-H. Lin, W.-X. Tu, W.-Y. Tsai, and T.-W. Cheng; Tang et al., Y.-Q. Tang, Y. Guang, Y. Li, M. Cai, and X.-L. Wu; Twing et al., K. Twing, J. Biddle, C. Martin, K. Lloyd, and A. Teske; Yeung et al., W. C. Yeung, K. Lee, S. Cobanli, T. King, J. Bugden, L. G. Whyte, and C. W. Greer; Zhang et al., L. Zhang, H. Dang, and X. Luan.