TABLE 3.
Depth intervals in which various mcrA clusters were detected using general and group-specific primersa
| Primer | Depth (cmbsf) |
No. of mcrA clusters (of 14) | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Methanoplanus | Methanomicrobiales Seep mcrA cluster | GB Methanomicrobiales mcrA cluster | Methanocaldococcus | Methanopyrus kandleri | Methanohalophilus | Methermicoccusb | ANME-1 | ANME-2 | Group e | DB Guaymas group II | DB Guaymas group III | Methanopyrus mcrA subcluster I | Methanopyrus mcrA subcluster II | ||
| ME1/ME2 | 0–1, 5–6 | 0–1, 5–6 | 0–1, 4–5 | 4–5 | 0–2, 4–5 | 0–1 | 0–2, 4–6 | 7 | |||||||
| mcrI | 0–1 | 0–1 | 0–1, 2–3, 4–5 | 5–6 | 2–3, 4–6 | 0–1 | 0–1, 2–3 | 0–1 | 0–1, 2–3 | 2–3 | 10 | ||||
| mcrIRD | 0–1 | 0–1, 5–6 | 0–3 | 1–2 | 0–1, 2–8 | 0–3 | 0–4 | 0–1 | 0–1 | 0–10 | 4–6 | 1–2 | 12 | ||
| mcrANME-1 | 0–2 | 1 | |||||||||||||
| mcrANME-2 | 0–3 | 1 | |||||||||||||
| mcrFCI | 1–2 | 1 | |||||||||||||
| mcrMmicrob | 0–4 | 1 | |||||||||||||
| mcrMcul | 2–3 | 1 | |||||||||||||
| mcrGMcul | 0–3, 5–6 | 1 | |||||||||||||
| mcrMbac | 0–1 | 0–2 | 0–1 | 3 | |||||||||||
| mcrMcoc | 1–2, 3–4 | 2–4 | 1 | ||||||||||||
| mcrMpK | 0–8 | 1 | |||||||||||||
| mcrDBGrII | 0–10 | 1 | |||||||||||||
| mcrDBGrIII | 0–6 | 2 | |||||||||||||
a
Only data for group-specific primer pairs that successfully amplified mcrA genes are shown.
b
To simplify, all three Methermicoccaceae genus level phylotypes treated as one cluster in this table.