TABLE 1.
Primers for amplifying the complete eight segments of ISAV and the PB2, PB1, PA, and NP ORF from ISAV901_09
| Segment primer | Primer sequencea (5′ to 3′) |
|
|---|---|---|
| Forward | Reverse | |
| 1a | AGCTAAGAATGGACTTTATATCAGAAAACACG | AACCTTCGAAGCCAAACAGATAG |
| 1b | CAATATCAAGTCCGTTCGACGTGG | AGTAAAAAATGGACATTTTATTGATTAAAAGTATCGTC |
| 2 | AGCAAAGAACGCTCTTTAATAACC | AGTAAAAAATGCTCTTTTACTTATTAAAAAT |
| 3 | AGCAAAGATTGCTCAAATCCC | AGTTAAAATTGCTCTTTTCTTTATTTG |
| 4 | AGCTAAGATTGGCTGTTTCAAGA | AGTAAAAATTGGCTTTTTGGAAAA |
| 5 | AGTTAAAGATGGCTTTTCTAACAATTTT | AGTAAAAATTGGCTATTTATACAATTAATAATG |
| 6 | AGCAAAGATGGCACGATTCA | AGTAAAAAATGCACTTTTCTGTAAACG |
| 7 | AGCTAAGATTCTCCTTCTACAATGGA | AGTAAAAATTCTCCTTTTCGTTTTAAA |
| 8 | AGCAAAGATTGGCTATCTACCA | AGTAAAAAAAGGCTTTTTATCTTTTG |
| PB2 | NcoI-ATGCCATGGACTTTATATCAGAAAACACGATCAGCG | XhoI-CCGCTCGAGAACACCATATTCATCCATAGG |
| PB1 | SmaI-TCCCCCGGGAAACTCTAGTAGGTG | XhoI-CCGCTCGAGAACACGCTTTTTCTTCTTAATCAC |
| NP | MluI-CGACGCGTCATGGCCGATAAAGGTATGAC | XbaI-CGCTCTAGATCAAATGTCAGTGTCTTCCTC |
| PA | NcoI-CATGCCATGGATAACCTCCGTGAATGCATAAACC | XhoI-CCGCTCGAGTTGGGTACTGACTGCAATTTTC |
a
The restriction sites are underlined.