TABLE 4.
Profiling of the dominant taxa encoding putative hemicellulasesa
Domain | Phylum | Genus | Species | Abundanceb (%) | Relative ratioc (%) | % reads with the following % identityd: |
|||
---|---|---|---|---|---|---|---|---|---|
<30 | ≥30–<60 | ≥60–<90 | ≥90 | ||||||
Bacteria | Firmicutes | Ruminococcus | Ruminococcus flavefaciens | 0.0172 | 14.6800 | 0 | 35 | 58 | 7 |
Ruminococcus albus | 0.0089 | 7.7819 | 0 | 22 | 63 | 15 | |||
Ruminococcus champanellensis | 0.0036 | 3.0999 | 0 | 37 | 60 | 3 | |||
Ruminococcus sp. | 0.0020 | 1.7640 | 0 | 23 | 73 | 4 | |||
Clostridium | Clostridium methylpentosum | 0.0017 | 1.5102 | 0 | 100 | 0 | 0 | ||
Eubacterium | Eubacterium cellulosolvens | 0.0027 | 2.3070 | 0 | 36 | 64 | 0 | ||
Bacteroidetes | Prevotella | Prevotella ruminicola | 0.0140 | 12.4335 | 0 | 15 | 78 | 7 | |
Prevotella bergensis | 0.0013 | 1.1137 | 0 | 14 | 79 | 7 | |||
Bacteroides | Bacteroides eggerthii | 0.0018 | 1.5771 | 0 | 15 | 85 | 0 | ||
Fibrobacteres | Fibrobacter | Fibrobacter succinogenes | 0.0073 | 6.4952 | 0 | 12 | 70 | 18 | |
Subtotal for 10 species | 0.0607 | 52.7623 | |||||||
Eukaryotes | Protozoa | Eudiplodinium | Eudiplodinium maggii | 0.0013 | 1.1806 | 0 | 37 | 73 | 0 |
Polyplastron | Polyplastron multivesiculatum | 0.0014 | 1.2248 | 0 | 73 | 27 | 0 | ||
Epidinium | Epidinium ecaudatum | 0.0012 | 0.9975 | 0 | 9 | 58 | 33 | ||
Subtotal for 3 species | 0.0040 | 3.4029 | |||||||
Total | 0.0646 | 56.1652 | |||||||
Unclassified uncultured organisms | 0.0110 | 9.6039 | 0 | 19 | 48 | 33 |
Average of data for the two cow samples are shown.
The abundance refers to the proportion of hemicellulase-related reads in the total non-rRNAs. Only organisms with hemicellulase-related reads with an abundance of ≥0.001% are shown.
The relative ratio refers to the proportion of the number of hemicellulase-related reads in a given organism in the total number of hemicellulase-related reads.
Similarities of proteins encoded by transcripts to that of known hemicellulases from a given organism at the amino acid sequence level are shown.