TABLE 3.
Identification and characterization of quorum-quenching ORFs derived from three bacterial isolates
| Bacterial isolate (isolate no.) | QQ-active fosmid clone (QQ activity) | Designation of QQ-ORFa (accession no.; MBP fusion plasmid) | Closest homolog (% identity) | QQ activity of MBP fusion proteinb |
|---|---|---|---|---|
| Photobacterium sp. (34) | 7/B4 (AHL + AI-2) | QQ-No.34a (JX306042; pRS622) | IcmF-like protein from Vibrio sp. (53) | AHL + AI-2 |
| QQ-No.34b (JX306043; pRS623) | ImpA-like protein from Vibrio sp. (45) | ND | ||
| Pseudoalteromonas sp. (16) | 4/B6 (AHL + AI-2) | QQ-No.16a (JX306044; pRS795) | Membrane transport protein vfu_B01239 from V. furnissii (76) | AHL + AI-2 |
| 4/C5 (AHL + AI-2) | QQ-No.16b (JX306045; pRS796) | Hypothetical protein vfu_B01276 from V. furnissii (87) | AHL + AI-2 | |
| QQ-No.16c (JX306046; pRS797) | Purine nucleoside phosphorylase from V. furnissii (92) | ND | ||
| QQ-No.16d (JX306047; pRS798) | Hypothetical protein vfu_B01063 from V. furnissii (75) | AHL + AI-2 | ||
| V. parahaemolyticus (10) | 9/H4 (AHL) | QQ-No.10a (JX306048; pRS799) | Hypothetical protein V12G01_07378 from V. alginolyticus (90) | AHL |
| QQ-No.10b (JX306049; pRS800) | Virulence-mediating protein VirC from V. alginolyticus (87) | ND | ||
| QQ-No.10c (JX306050; pRS801) | Hypothetical protein V12G01_06641 from V. alginolyticus (90) | AHL | ||
| 9/H3 (AHL) | QQ-No.10d (JX306051; pRS802) | Putative permease from V. alginolyticus (96) | ND | |
| QQ-No.10e (JX306052; pRS803) | Sigma cross-reacting protein 27A from V. alginolyticus (90) | ND |
a
The QQ-ORFs identified by subcloning and/or transposon mutagenesis were characterized after heterologous expression of the respective MBP fusion proteins.
b
The QQ activities of the MBP fusion proteins were analyzed using reporter strains AI1-QQ.1 and AI2-QQ.2. ND, not detectable.