. 2015 Jan 30;81(4):1387–1396. doi: 10.1128/AEM.03028-14

TABLE 1.

Plasmid and constructs used in this study

Name	Relevant characteristic(s)^a	Source or reference
pAZIL	Shuttle cloning vector, 7,109 bp, Em^r	28
pAZIL-BH18.5	18,466-bp BamHI fragment from pCG1 cloned in BamHI site of pAZIL, Em^r	This work
pAZIL-BH3	3,018-bp BamHI fragment from pCG1 cloned in BamHI site of pAZIL, Em^r	This work
pAZIL-Bg4.27	4,266-bp BglII fragment from pCG1 cloned in BglII site of pAZIL, Em^r	This work
pAZIL-E650	656-bp EcoRI fragment from pCG1 cloned in EcoRI site of pAZIL, Em^r	This work
pAZIL-BHBc1.3	1,328-bp BamHI/BclI fragment from pCG1 cloned in BamHI/BclI site of pAZIL, Em^r	This work
pAZIL-BgBH1	996-bp BglII/BamHI fragment from pCG1 cloned in BglII/BamHI site of pAZIL, Em^r	This work
pGhost9	Em^r, vector for insertional mutagenesis with a temperature-sensitive origin of replication	31
pGhost/31	pGhost9 derivative carrying part of the orf2 gene	This work
pGhost/32	pGhost9 derivative carrying part of the rfbB gene	This work
p-GEM-T-Easy	3,015 bp, Amp^r, PCR cloning vector	Promega

Em^r, resistance to erythromycin; Amp^r, resistance to ampicillin.