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. 2015 Jan 23;17(1):79–88. doi: 10.1016/j.neo.2014.11.004

Figure 3.

Figure 3

The 5'UTR of CLOCK mRNA interacts with HULC via complementary base pairing in hepatoma cells. (A) A model shows the complementary region between HULC and CLOCK 5'UTR. Schematic diagram exhibits the generated mutant site at the CLOCK 5'UTR in complementary region binding to HULC, which is inserted in pGL3-control vector. (B) The effect of HULC on reporters of pGL3-CL-5'UTR (or pGL3-CL-5'UTR-mut) was examined by luciferase reporter gene assays in L-O2 cells. (C) The effect of HULC siRNA on reporters of pGL3-CL-5'UTR (or pGL3-CL-5'UTR-mut) was measured by luciferase reporter gene assays in HBx-elevated HULC-highly-expressing L-O2-X cells. (D and E) The experiments above were repeated in HepG2 and HepG2.2.15 cells. Statistically significant differences are indicated: **P < .01, no significance (NS); Student’s t test.