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. 2015 Jan 23;17(1):141–151. doi: 10.1016/j.neo.2014.12.002

Figure 3.

Figure 3

RhoC is expressed in HNSCC and is activated by EGFR. (A) Whole cell lysate of HOK, UM-SCC-(1, 5, 6, 10B, 17B), and SCC-(0, 1483) were separated by PAGE and blotted with RhoA and RhoC antibodies. GAPDH was used as loading control. (B) HOK and UM-SCC-6 cells were stimulated with 100 ng/ml of EGF for varying durations from 5 minutes to 2 hours and RhoA and RhoC activation was evaluated by a pull down assay. Total RhoA, RhoC and GAPDH were used as loading controls. Active, GTP-bound Rho was quantified by densitometry and normalized to total normalized Rho with respect to GAPDH.