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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Biol Psychiatry. 2014 Jul 28;79(5):362–371. doi: 10.1016/j.biopsych.2014.07.019

Figure 2. Histone H3 phosphorylation at Ser10 is decreased in mitogen- and stress-activated kinase 1 knock out (MSK1 KO) mice.

Figure 2

Wild-type and MSK1 KO mice received unilateral striatal injections of 6-hydroxydopamine (Les) while the contralateral striatum remained intact (Unles) and were treated for 9 days with L-DOPA. A, Representative confocal micrographs of phospho-MSK1 (Thr581) (P-MSK1) determined by immunofluorescence in the striata of wild-type and MSK1 KO mice 30 min after the last administration of L-DOPA. Note the absence of P-MSK1 in MSK1 KO mice. Scale bar = 40 μm. B-D, Phospho-Ser10-histone H3 (P-H3; B), phospho-Thr34-DARPP-32 (P-DARPP-32; C) and phospho-Thr202/Tyr204-ERK2 (P-ERK2; D) were determined 30 min after the last administration of L-DOPA by Western blotting. Top: representative autoradiograms obtained using antibodies against phosphorylated proteins, total proteins, and β-actin or GAPDH as loading controls. Bottom: summary of data calculated as percent of control (wild-type unlesioned hemisphere) and shown as means ± SEM. * p < 0.05, *** p < 0.001 vs. unlesioned hemisphere of the same genotype; ○○○ p < 0.001 vs. wild-type lesioned hemisphere.

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