Figure 1.

A schematic illustration of the synthesis of 800ZW–SPION@dSiO₂–YY146.

Notes: Uniform dSiO₂-coated SPION nanoparticles (1) were first modified with –NH₂ groups to form dSiO₂NH₂. (2) dSiO₂–NH₂ was labeled with 800ZW for NIRF imaging and subsequent –NH₂ to PEG; (3) afterward, PEGylated with SCM-PEG5k-Mal to form 800ZW–SPION@dSiO₂–PEG; (4) which was then subjected to mAb conjugation to form 800ZW–SPION@dSiO₂–YY146.

Abbreviations: SPION, superparamagnetic iron oxide nanoparticles; NIRF, near infra-red fluorescence; PEG, polyethylene glycol; mAb, monoclonal antibody; APS, ammonium persulfate.