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. 2015 Jan 20;10:749–763. doi: 10.2147/IJN.S62837

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© 2015 Wang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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In vitro investigation of 800ZW–SPION@dSiO₂–YY146 conjugation.

Notes: FACS analysis of 800ZW–SPION@dSiO₂–YY146 conjugation in MKN45 (CD146 positive) cells after 30 minutes incubation and subsequent washing. (A) Whole cells FACS distribution; (B) analysis gate for the single cells. (C) Control group: cells are all located at the NIRF channel negative. (D) Target group: 40.08% cells are labeled by NIRF dye; (E) blocking group: the labeled cells drop to 7.532% after YY146 blocking; (F) negative group: 17.4839% labeling in the negative group.

Abbreviations: SPION, superparamagnetic iron oxide nanoparticles; FACS, fluorescence activated cell sorter; NIRF, near infra-red fluorescence; SSC, side scatter; FSC, forward angle light scatter; FITC, fluorescein isothiocyanate.