Figure 5.

Confocal microscopic image of 800ZW–SPION@dSiO₂–YY146.

Notes: In vitro investigation of nanoparticle binding affinities of CD146 on MKN45 cells: cells were incubated with (A) 70 μL of 5 nmol/mL 800ZW–SPION@dSiO₂–YY146, targeting group (B) YY146 (200 μg ×3) blocking and 70 μL of 5 nmol/mL 800ZW–SPION@dSiO₂–YY146, (C) 70 μL of 5 nmol/mL 800ZW–SPION@dSiO₂. Strong staining of cell membrane was observed in targeting group (A) whereas background levels were seen in blocking (B) and control (C) (40× oil).

Abbreviations: SPION, superparamagnetic iron oxide nanoparticles; DAPI, 4′,6-diamidino-2-phenylindole.