Figure 2.

Effect of chloroquine (CQ) and antibiotic treatment on Staphylococcus aureus infection-induced alteration in H₂O₂ release in the lysate and supernatant in murine peritoneal macrophages. The lysate (A) and supernatant (sup) (B) recovered after time-dependent phagocytosis in the presence or absence of chloroquine or antibiotics were used to determine the H₂O₂ release.

Notes: H₂O₂ release is expressed in terms of μM/10⁶ cells. Results are presented as mean ± standard deviation of three independent experiments. *significant difference with respect to CM; ^#significant difference with respect to SAM; ^{^}significant difference with respect to CQ plus S. aureus-infected macrophages at the P<0.05 level of significance; ^$significant difference with respect to either ciprofloxacin (CIP) or azithromycin (AZM) plus S. aureus-infected macrophages.

Abbreviations: CM, control macrophages; CQ + SAM, chloroquine-pretreated + S. aureus-infected macrophages; CQ + SAM + AZM, chloroquine-pretreated macrophages infected with S. aureus and then treated with azithromycin; CQ + SAM + CIP, chloroquine-pretreated macrophages infected with S. aureus and then treated with ciprofloxacin; SAM, S. aureus-infected macrophages; SAM + AZM, S. aureus-infected macrophages treated with azithromycin; SAM + CIP, S. aureus-infected macrophages treated with ciprofloxacin.