levels of (A) interferon gamma (IFN-γ) and (B) tumor necrosis factor alpha (TNF-α) in the supernatants collected from Staphylococcus aureus-infected macrophages in the presence or absence of chloroquine (CQ) or antibiotics were determined utilizing enzyme-linked immunosorbent assay according to the manufacturer’s recommendations.
Notes: Results from triplicate experiments are presented. *significant difference with respect to CM; #significant difference with respect to SAM; ^significant difference with respect to CQ plus S. aureus-infected macrophages at the P<0.05 level of significance; $significant difference with respect to either ciprofloxacin (CIP) or azithromycin (AZM) plus S. aureus-infected macrophages.
Abbreviations: CM, control macrophages; CQ + SAM, chloroquine-pretreated plus S. aureus-infected macrophages; CQ + SAM + AZM, chloroquine-pretreated macrophages infected with S. aureus and then treated with azithromycin; CQ + SAM + CIP, chloroquine-pretreated macrophages infected with S. aureus and then treated with ciprofloxacin; SAM, S. aureus-infected macrophages; SAM + AZM, S. aureus-infected macrophages treated with azithromycin; SAM + CIP, S. aureus-infected macrophages treated with ciprofloxacin.