Table 1.
effect of chloroquine (CQ) and antibiotic treatment on S. aureus infection-induced lysozyme enzyme activity in the supernatant and lysate by murine peritoneal macrophages
| Group | Lysozyme enzyme activity in supernatant, units/mg of protein (mean ± SD)
|
Lysozyme enzyme activity in lysate, units/mg of protein (mean ± SD)
|
||||
|---|---|---|---|---|---|---|
| 30 min | 60 min | 90 min | 30 min | 60 min | 90 min | |
| CM | 14.728±0.772 | 17.350±0.660 | 46.746±1.960 | 10.860±0.550 | 12.000±3.080 | 7.600±1.980 |
| SAM | *231.450±7.930 | *166.780±2.240 | *104.540±4.320 | *165.670±6.130 | *224.930±4.000 | *254.060±5.040 |
| CQ + SAM | #166.780±2.240 | #120.610±5.350 | #81.240±1.800 | #100.485±0.064 | #93.930±1.680 | #66.040±5.790 |
| SAM + AZM | #126.470±0.898 | #103.479±8.710 | 86.160±5.340 | #73.770±1.870 | #93.930±1.600 | #159.205±3.820 |
| SAM + CIP | #103.370±8.710 | #83.390±4.40 | #55.380±2.250 | #70.915±10.760 | #85.690±7.000 | #101.417±7.570 |
| CQ + SAM + AZM | $167.970±2.480 | $128.430±0.890 | ^,$34.740±3.510 | ^,$337.000±27.000 | ^,$212.640±0.960 | ^,$26.520±6.630 |
| CQ + SAM + CIP | ^,$77.980±2.680 | ^,$49.685±5.490 | ^,$29.110±2.460 | ^,$219.030±5.220 | ^,$165.790±4.410 | ^110.900±0.800 |
Notes: The supernatants and cell-free lysates obtained in the phagocytosis assay were allowed to interact with a suspension of Micrococcus lysodeikticus in 0.15 M potassium phosphate buffer (ph 6.2, optical density fixed at 0.6–0.7 at 450 nm). One unit of lysozyme was defined as the amount of enzyme that produces a decrease in absorbance of 0.001/minute at 450 nm and the lysozyme enzyme activity is expressed in terms of units/mg protein. Results are presented as the mean ± standard deviation of three independent experiments.
significant difference with respect to CM;
significant difference with respect to SAM;
significant difference with respect to CQ plus S. aureus-infected macrophages at the P<0.05 level of significance;
significant difference with respect to either ciprofloxacin (CIP) or azithromycin (AZM) plus S. aureus-infected macrophages.
Abbreviations: CM, control macrophages; CQ + SAM, chloroquine-pretreated plus S. aureus-infected macrophages; CQ + SAM + AZM, chloroquine-pretreated macrophages infected with S. aureus and then treated with azithromycin; CQ + SAM + CIP, chloroquine-pretreated macrophages infected with S. aureus and then treated with ciprofloxacin; SAM, S. aureus-infected macrophages; SAM + AZM, S. aureus-infected macrophages treated with azithromycin; SAM + CIP, S. aureus-infected macrophages treated with ciprofloxacin.