Figure 5.

Subcellular localization of hypericin (HY) in THP-1 macrophages.

Notes: (A) Colocalization of HY and nuclei. The cells were stained with 4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI) to visualize nuclei (shown in blue). The natural fluorescence of HY (shown in red) was used to image its presence in the cells (scale bar, 0.05 mm). (B) Colocalization of HY and mitochondria. The cells were stained with Mito-Tracker Green to visualize mitochondria (shown in green) and with DAPI to visualize nuclei (shown in blue; scale bar, 0.05 mm).