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. 2014 Nov 24;5:1–7. doi: 10.1016/j.fob.2014.11.006

Fig. 1.

Fig. 1

Overexpression of sll1545 in E. coli and purification of the recombinant protein on Ni–NTA agarose. (A) SDS–PAGE analysis of cell lysate showing overexpression of sll1545 and the purified protein. Lanes 1–4 represent molecular weight markers, supernatant of un-induced culture lysate, supernatant of induced culture lysate and purified protein, respectively. (B) Molecular weight and subunit structure of sll1545. SEC profile of sll1545 on Superdex™ 200 10/300 GL column at pH 8.0 and 25 °C.