Fig. 5.

Effect of PI3K inhibitor on EPS-stimulated GSH synthesis and Nrf2 activation in BAECs. BAECs were pretreated with 20 µM LY294002 (LY) for 3 h and then treated with 50 µM EPS. GCLM mRNA (A), intracellular GSH (B) levels, and nuclear levels of active Nrf2 (C) were measured after 4 h, 24 h, and 4 h treatment, respectively. Values are means±SD of three experiments. *Significant difference from the value of control (P<0.05). (C) COS-7 cells (Nrf2 transfected) nuclear extracts provided by the manufacturer were used as a positive control; the value was 2.0±0.3.