Fig. 6.

Effect of EPS on oxidative stress in BAECs. BAECs were pretreated with 50 µM EPS for 16 h. Subsequently, the untreated or EPS-treated cells were exposed to 100 µM H₂O₂ (A) and 50 µM tert-butylhydroperoxide (t-BHP) (B) for 24 h. Cell viability was assessed by measuring acid phosphatase activity. Values are means±SD of six experiments. (C) BAECs were pretreated with 50 µM EPS and 20 µM LY294002 (LY) and then exposed to 50 µM t-BHP for 4 h. Oxidative damage to mitochondria was estimated by using MitoTracker Red CMXRos and a confocal microscope. (D) Quantitative analysis of data shown in C. Values are means±SD of three experiments. Significant differences between the compared groups are indicated: *P<0.05, **P<0.05, and ***P<0.05 versus control, oxidizing agent alone, and oxidizing agent and EPS, respectively.