Fig. 6.

MMP2-dependent nSMase2 activation by H₂O₂ involves p38MAPK. Wt mFbl, MMP2^−/− (A) and fro/fro fibroblasts (B) were stimulated with H₂O₂ at the indicated times. The phosphorylation of p38MAPK was evaluated by western-blot. Alternatively, nSMase activity was measured in wt mFbl incubated with H₂O₂w/wo the SB203580 (10 µM) (C). These experiments were performed in triplicate. ^⁎, P <0.05 vs. basal.