Fig. 7.

The phosphorylation of Src and AnxA2 depends on the activation of nSMase2, via MMP2 and p38MAPK. Cells were stimulated by H₂O₂ at the indicated times, and src phosphorylation was determined on wt and MMP2^−/− (A), and on fro/fro mFbl (B). In C, effect of the p38MAPK inhibition by H₂O₂ in src^k+ and src^kd fibroblasts, determined using the specific fluorogenic assay as reported in the experimental section. (E) Effect of SB203580 on AnxA2 phosphorylation in wt mFbl. (F) AnxA2 phosphorylation by H₂O₂ in MMP2^−/− fibroblasts. Data are means±SEM from at least three independent experiments and are expressed relative to basal. ^⁎, P<0.05 vs. basal.