Fig. 5.

Effects of fatty acids treatment and/or CoCl₂ on histone H2AX. Cells were incubated with 1 mM fatty acid mixture (oleate/palmitate, 2/1; black bars) or vehicle in which fatty acids were dissolved (white bars) for 24 h. Afterwards, cells were treated with or without 100 µM CoCl₂ for 4 h. (A) Representative SDS PAGE gels and (B) total and (C) phosphorylation status of histone 2AX (H2AX) at Ser139. (D) H2AX total to phosphorylated ratio. Results are means±SE. Means with different letters in each group differ at p<0.05. Con, control; FA, fatty acids.