Table 1.
Total content of cytokinins in transgenic lines and wild type (ng/g fresh weight)
| Line | Cytokinins | ||||||
|---|---|---|---|---|---|---|---|
| Young leaf | Mature leaf | Stem shoot | Flower bud | Ovule | Total | Increase level (%) | |
| WT | 75.5 | 32.6 | 142.5 | 38.5 | 983.3 | 1,272.4 | – |
| CR-3 | 85.8 | 65.0 | 190.3 | 53.0 | 1,138.2 | 1,532.3 | 20.4 |
| CR-6 | 96.8 | 67.4 | 208.0 | 70.1 | 1,536.9 | 1,979.2 | 55.5 |
| CR-13 | 135.7 | 96.0 | 229.6 | 160.0 | 2,359.1 | 2,980.4 | 134.2 |
Well-ground samples were extracted in cold 80 % (v/v) methanol. Cytokinins were purified by a Sep-Pak® Plus tC18 cartridge (Waters, Ireland) and determined by HPLC–MS/MS systems (ABsciex, USA). The total values were the sum of cytokinin contents in young leaf, mature leaf, stem shoot, flower bud, and ovule, respectively. WT wild type; CR-3, CR-6 and CR-13, 35S::GhCKXRNAi transgenic plants