Table 2.
In vitro inhibition of proteases and lipoxygenase by heterologous expressed potato tuber protease inhibitors
| Inhibitor type | Inhibitor ID | Trypsin EC3.4.21.4a | Pig pancreas elastase EC3.4.21.36 | BACE (β-secretase) EC3.4.23.46 | Cathepsin K EC3.4.22.38 | 5-Lipoxygenase EC1.13.11.34 | HIV-1 protease EC3.4.23.16 |
|---|---|---|---|---|---|---|---|
| KTI-A | PI9070 | 9 | – | – | – | – | – |
| KTI-A | PI4063 | 6 | – | – | – | – | – |
| KTI-B | PI4435 | 62 | – | – | – | – | – |
| KTI-B | PI4587 | 53 | – | + (IC50 0.81 µM) | – | – | – |
| KTI-B | PI8234 | 59 | – | + (IC50 0.4 µM) | – | – | – |
| KTI-B | PI2112 | 65 | – | + (IC50 0.36 µM) | – | – | – |
| KTI-B | PI2568 | 55 | – | – | – | – | – |
| KTI-B | PI5887 | 38 | – | + (IC50 0.47 µM) | – | – | – |
| KTI-B | PI8383 | 61 | – | – | – | – | – |
| KTI-B | PI5918 | 83 | – | – | – | – | – |
| KTI-B | PI8311 | 16 | – | – | – | – | – |
| KTI-B | PI9142 | 53 | – | – | – | – | – |
| KTI-C | PI1410 | 0 | – | – | + (IC50 0.09 µM) | + (IC50 1.59 µM) | – |
| KTI-C | PI5446 | 5 | – | + (IC50 0.72 µM) | + (IC50 0.08 µM) | + (IC50 1.01 µM) | – |
| KTI-C | PI4202 | 5 | – | – | + (IC50 0.02 µM) | + (IC50 1.28 µM) | – |
| PIN I | PI6013 | 44 | – | – | + (IC50 0.77 µM) | – | + (IC 50 0.67 µM) |
| PIN II | PI4434 | 43 | + (IC50 0.67 µM) | – | – | – | – |
| PIN II | PI6669 | 87 | + (IC50 0.97 µM) | – | – | – | – |
| PMEI | PI7531 | 10 | – | – | – | – | – |
a % Inhibition at a molar ratio 2:1 of inhibitor: trypsin