Figure 5. Altered activity of KATP channels involved in potassium transport.
(A) Western blots of membrane enriched lysate (that lacks mitochondria, peroxisomes, and lysosomes) from 2-month old wild-type and heterozygous Ryr1AG/+ soleus muscle analyzed for KATP6.1, KATP6.2, and KIR2.1. (B) Quantification of protein levels from Western blots of membrane enriched lysates. Each sample was first normalized to its own loading control and then the values from mutant and wild-type on the same blot were compared. Statistical analyses were determined from a minimum of 3 blots and at least two independent samples (n = 4 for each Western). (C) KATP current densities of isolated soleus muscle fibers from 2-month old wild-type and Ryr1AG/+ littermates with and without glibenclamide. Numbers on top of bars are number of fiber recordings. (D–E) Representative current density recordings with the same tip resistance from Ryr1+/+ and Ryr1AG/+ soleus muscle fibers (E) with and (D) without 2 μM glibenclamide.