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. 2015 Jan 7;4:e02923. doi: 10.7554/eLife.02923

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© 2015, Hanson et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 6. — (A) Normalized internal potassium concentrations of soleus muscle from 2-month old Ryr1^+/+ and Ryr1^AG/+ mice fed 0.6% K⁺ diet for 4 weeks, then placed on 5.2% K+ diet or 0.6% diet + enalapril for 4 weeks, and then bath exposed to different extracellular potassium concentrations. (B, C) Average grip strength (B, five trials/mouse and 5 mice/set; p values <0.001) and in vivo hanging task (C, 10 trials/mouse, n = 5 per set; p values <0.001) assayed from 2-month old Ryr1^+/+ (black bar) and Ryr1^AG/+ (grey bar) mice maintained for 4 weeks on control 0.6% K⁺ diet, 5.2% K⁺ diet, or 0.6% K⁺ diet supplemented with enalapril. (D) Quantification of number of internalized nuclei per 100 myofibers in 12-month old mice or in 2-month old mice maintained for 4 weeks on control 0.6% K⁺ diet, 5.2% K⁺ diet, or 0.6% K⁺ diet supplemented with enalapril (n = 10 per muscle, n = 3 per set of muscles for total of n = 30; p values <0.001). (E–P) Vastus lateralis myofibers from 2-month old Ryr1^+/+ and Ryr1^AG/+ mice maintained for 4 weeks on control 0.6% K⁺ diet, 5.2% K⁺ diet, or 0.6% K⁺ diet supplemented with enalapril. Cross-sections stained with H&E (left panels) and COX (right panels). Ryr1^AG/+ mice on 5.2% K diet show increased COX staining and no internalized nuclei, similar to Ryr1^+/+. These pathological features are still observed in Ryr1^AG/+ mice on 0.6% K⁺ diets. Enalapril increases COX staining but some internalized nuclei are observed, even in Ryr1^+/+. Scale bar = 50 μm; error bars as standard error of the mean (SEM).

DOI: http://dx.doi.org/10.7554/eLife.02923.012

Figure 6—figure supplement 1. — (A) Normalized internal potassium concentrations of soleus muscle from 2-month old Ryr1^+/+ and Ryr1^AG/+ mice fed 0.6% K⁺ diet for 4 weeks, then placed on 5.2% K+ diet or 0.6% diet + enalapril for 4 weeks, and then bath exposed to different extracellular potassium concentrations. (B, C) Average grip strength (B, five trials/mouse and 5 mice/set; p values <0.001) and in vivo hanging task (C, 10 trials/mouse, n = 5 per set; p values <0.001) assayed from 2-month old Ryr1^+/+ (black bar) and Ryr1^AG/+ (grey bar) mice maintained for 4 weeks on control 0.6% K⁺ diet, 5.2% K⁺ diet, or 0.6% K⁺ diet supplemented with enalapril. (D) Quantification of number of internalized nuclei per 100 myofibers in 12-month old mice or in 2-month old mice maintained for 4 weeks on control 0.6% K⁺ diet, 5.2% K⁺ diet, or 0.6% K⁺ diet supplemented with enalapril (n = 10 per muscle, n = 3 per set of muscles for total of n = 30; p values <0.001). (E–P) Vastus lateralis myofibers from 2-month old Ryr1^+/+ and Ryr1^AG/+ mice maintained for 4 weeks on control 0.6% K⁺ diet, 5.2% K⁺ diet, or 0.6% K⁺ diet supplemented with enalapril. Cross-sections stained with H&E (left panels) and COX (right panels). Ryr1^AG/+ mice on 5.2% K diet show increased COX staining and no internalized nuclei, similar to Ryr1^+/+. These pathological features are still observed in Ryr1^AG/+ mice on 0.6% K⁺ diets. Enalapril increases COX staining but some internalized nuclei are observed, even in Ryr1^+/+. Scale bar = 50 μm; error bars as standard error of the mean (SEM).

DOI: http://dx.doi.org/10.7554/eLife.02923.012