Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jan 29;5:8124. doi: 10.1038/srep08124

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015, Macmillan Publishers Limited. All rights reserved

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A,D) Left, Montages of time-lapse TIRF images for an assembling (panel A) and disassembling (panel D) adhesion (white arrows) in cells expressing GFP-paxillin are shown in pseudo-color coding, which indicates the range of fluorescence intensities. Cool colors correspond to lower intensity values, and warm colors correspond to higher intensity values. Right, Outlines of the adhesions as segmented by PAASTA. (B,E) Graphs of the fluorescence intensities of the assembling (panel B) and disassembling (panel E) adhesion are shown for both Manual and PAASTA tracking. (C,F) Plots of the natural log of the fluorescence intensity of the adhesions at given time points (I) relative to the initial fluorescence intensity (I₀) are shown. Trendlines with the corresponding equation (y = mx + b) and R² values are shown for fluorescence intensities that were obtained manually (Manual) and with PAASTA. The slopes of these graphs (m) are the apparent rate constant for adhesion assembly (panel C) or the rate constant for adhesion disassembly (panel F). (G) The average t_1/2 values for adhesion assembly and disassembly for cells expressing Spec-Pax or Spec-Vinc are shown for Manual and PAASTA adhesion tracking. S.E.M. was calculated from: 20–21 adhesions (10–11 assembly, 10 disassembly) for each construct. A total of 14 cells were analyzed for adhesion assembly and disassembly. A Wilcoxon rank sum test showed no statistically significant difference between Manual and PAASTA tracking for Spec-Pax assembly (Z = −1.682, p = 0.093), disassembly (Z = −0.153, p = 0.878); or Spec-Vinc assembly (Z = −1.112, p = 0.266), disassembly (Z = −0.255, p = 0.799). For panels A and D, Bar, 1 μm.