Table 6. Validation of microarray results by qPCR.
| Liver | ||||
|---|---|---|---|---|
| Microarray | qPCR | |||
| FC | p | FC | p | |
| fads2d6 | +2.65 | 0.001 | +3.07 | 0.001 |
| fads2d5 | +2.91 | 0.002 | +3.96 | 0.021 |
| elovl2 | +1.55 | 0.010 | +1.69 | 0.267 |
| elovl5a | +1.51 | 0.014 | +1.13 | 0.716 |
| elovl5b | +1.47 | 0.020 | +1.29 | 0.688 |
| palld | +1.18 | 0.006 | +1.19 | 0.763 |
| ap1m2 | +1.36 | 0.010 | +1.28 | 0.817 |
| arsb | −1.71 | 0.000 | +1.11 | 0.516 |
| litaf | +1.52 | 0.043 | +1.04 | 0.404 |
| g3bp | +1.84 | 0.040 | +1.34 | 0.883 |
Data shown are the fold-changes (FC) between expression levels in fish fed diets FO and ECO and p value (assessed by REST 2009).fads2d6, delta-6 fatty acyl desaturase; fads2d5, delta-5 fatty acyl desaturase; elovl2, fatty acyl elongase 2; elovl5a, fatty acyl elongase 5 isoform a; elovl5b, fatty acyl elongase isoform b; palld, pallidin; ap1m2, AP-1 complex subunit mu-2; arsb, arysulfatase B; litaf, lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog; g3bp, galectin-3-binding protein.