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. 1977 May;74(5):1807–1810. doi: 10.1073/pnas.74.5.1807

Lipid-protein interaction in the phosphatidylcholine exchange protein.

P F Devaux, P Moonen, A Bienvenue, K W Wirtz
PMCID: PMC431009  PMID: 194240

Abstract

Incorporation of 2-acyl spin-labeled lecithin into the phosphatidylcholine protein from bovine liver results in an immobilization of the spin-label at the methyl and the carboxyl terminal end of the acyl chain. The nitroxide group on the protein-bound lecithin molecule is not accessible to ascorbate. This suggests that lecithin is buried in a pocket on the protein, which effectively shields the acyl chains from the medium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DAVIDSON F. M., LONG C. The structure of the naturally occurring phosphoglycerides. 4. Action of cabbage-leaf phospholipase D on ovolecithin and related substances. Biochem J. 1958 Jul;69(3):458–466. doi: 10.1042/bj0690458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Demel R. A., Wirtz K. W., Kamp H. H., Geurts van Kessel W. S., van Deenen L. L. Phosphatidylcholine exchange protein from beef liver. Nat New Biol. 1973 Nov 28;246(152):102–105. doi: 10.1038/newbio246102a0. [DOI] [PubMed] [Google Scholar]
  3. Devaux P. F., Bienvenüe A., Lauquin G., Brisson A. D., Vignais P. M., Vignais P. V. Interaction between spin-labeled acyl-coenzyme A and the mitochondrial adenosine diphosphate carrier. Biochemistry. 1975 Mar 25;14(6):1272–1280. doi: 10.1021/bi00677a028. [DOI] [PubMed] [Google Scholar]
  4. Hemminga M. A., Post J. F. Lipid-protein interactions in model membranes from bovine brain white matter. An ESR spin label and electron microscopy study. Biochim Biophys Acta. 1976 Jun 4;436(1):222–231. doi: 10.1016/0005-2736(76)90233-9. [DOI] [PubMed] [Google Scholar]
  5. Hesketh T. R., Smith G. A., Houslay M. D., McGill K. A., Birdsall N. J., Metcalfe J. C., Warren G. B. Annular lipids determine the ATPase activity of a calcium transport protein complexed with dipalmitoyllecithin. Biochemistry. 1976 Sep 21;15(19):4145–4151. doi: 10.1021/bi00664a002. [DOI] [PubMed] [Google Scholar]
  6. Hsia J. C., Piette L. H. Spin-labeling as a general method in studying antibody active site. Arch Biochem Biophys. 1969 Jan;129(1):296–307. doi: 10.1016/0003-9861(69)90179-9. [DOI] [PubMed] [Google Scholar]
  7. Hubbell W. L., McConnell H. M. Molecular motion in spin-labeled phospholipids and membranes. J Am Chem Soc. 1971 Jan 27;93(2):314–326. doi: 10.1021/ja00731a005. [DOI] [PubMed] [Google Scholar]
  8. Hubbell W. L., McConnell H. M. Motion of steroid spin labels in membranes. Proc Natl Acad Sci U S A. 1969 May;63(1):16–22. doi: 10.1073/pnas.63.1.16. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jost P. C., Capadil R. A., Vanderkooi G., Griffith O. H. Lipid-protein and lipid-lipid interactions in cytochrome oxidase model membranes. J Supramol Struct. 1973;1(4):269–280. doi: 10.1002/jss.400010404. [DOI] [PubMed] [Google Scholar]
  10. Jost P., Griffith O. H., Capaldi R. A., Vanderkooi G. Identification and extent of fluid bilayer regions in membranous cytochrome oxidase. Biochim Biophys Acta. 1973 Jun 22;311(2):141–152. doi: 10.1016/0005-2736(73)90261-7. [DOI] [PubMed] [Google Scholar]
  11. Kamp H. H., Sprengers E. D., Westerman J., Wirtz K. W., Van Deenen L. L. Action of phospholipases on the phosphatidylcholine exchange protein from beef liver. Biochim Biophys Acta. 1975 Sep 19;398(3):415–423. doi: 10.1016/0005-2760(75)90192-7. [DOI] [PubMed] [Google Scholar]
  12. Morrisett J. D., Pownall H. J., Gotto A. M., Jr Bovine serum albumin. Study of the fatty acid and steroid binding sites using spin-labeled lipids. J Biol Chem. 1975 Apr 10;250(7):2487–2494. [PubMed] [Google Scholar]
  13. Rousselet A., Colbeau A., Vignais P. M., Devaux P. F. Study of the transverse diffusion of spin-labeled phospholipids in biological membranes. II. Inner mitochondrial membrane of rat liver: use of phosphatidylcholine exchange protein. Biochim Biophys Acta. 1976 Mar 19;426(3):372–384. doi: 10.1016/0005-2736(76)90383-7. [DOI] [PubMed] [Google Scholar]
  14. Rousselet A., Guthmann C., Matricon J., Bienvenue A., Devaux P. F. Study of the transverse diffusion of spin labeled phospholipids in biological membranes. I. Human red bloods cells. Biochim Biophys Acta. 1976 Mar 19;426(3):357–371. doi: 10.1016/0005-2736(76)90382-5. [DOI] [PubMed] [Google Scholar]
  15. Wirtz K. W., Kamp H. H., van Deenen L. L. Isolation of a protein from beef liver which specifically stimulates the exchange of phosphatidylcholine. Biochim Biophys Acta. 1972 Aug 9;274(2):606–617. doi: 10.1016/0005-2736(72)90207-6. [DOI] [PubMed] [Google Scholar]
  16. van den Besselaar A. M., Helmkamp G. M., Jr, Wirtz K. W. Kinetic model of the protein-mediated phosphatidylcholine exchange between single bilayer liposomes. Biochemistry. 1975 May 6;14(9):1852–1858. doi: 10.1021/bi00680a008. [DOI] [PubMed] [Google Scholar]

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