Figure 7.

Influence of i n vitro leaf extract on AGS cell growth inhibition. a- Inhibitory effect of in vitro methanolic leaf extract on AGS cell proliferation was measured by MTT assay. IC-50 value of the extract on the cell viability was identified as 118.85 μg/mL. b – DNA cell cycle analysis of control and treated AGS cells. Cells were stained with PI to determine cell cycle phase distribution based on fluorescence of DNA. c - Apoptosis level was examined by flow cytometry for control and treated AGS cells. Cells were treated with methanolic extract of in vitro grown leaf tissue (IC50 - 118 μg/mL) for 24 h. Data are representative of three similar experiments.