Table 3.

Number of transcripts found in in vitro roots and leaves putatively encoding enzymes involved in withanolide biosynthesis

Enzymes	Abbreviation	Enzyme commission number	No. of transcripts
			Total	Expressed in both adventitious roots and leaves	Uniquely expressed in adventitious roots
1-Deoxy-D-xylulose-5-phosphate synthase	DXPS	2.2.1.7	8	8	0
1-Deoxy-D-xylulose-5-phosphate reductoisomerase	DXPR	1.1.1.267	3	3	0
2-C-Methyl-D-erythritol 4-phosphate cytidylyltransferase	MEP-CT	2.7.7.60	2	2	0
4-(Cytidine 5′-diphospho)-2-C-methyl-D-erythritol kinase	CDP-MEK	2.7.1.148	3	2	1
2-C-Methyl-D-erythritol 2,4-cyclodiphosphate synthase	MECDPS	4.6.1.12	1	1	0
(E)-4-Hydroxy-3-methylbut-2-enyl-diphosphate synthase	HMBPPS	1.17.7.1	1	1	0
4-Hydroxy-3-methylbut-2-enyl diphosphate reductase	HMBPPR	1.17.1.2	1	1	0
Isopentenyl-diphosphate delta-isomerase	IDI	5.3.3.2	4	4	0
Acetyl-CoA C-acetyltransferase	ACAT	2.3.1.9	19	12	7
Hydroxymethylglutaryl-CoA synthase	HMGCS	2.3.3.10	5	5	0
Hydroxymethylglutaryl-CoA reductase	HMGR	1.1.1.88	11	11	0
Hydroxymethylglutaryl-CoA reductase	HMGR	1.1.1.34	9	9	0
Mevalonate kinase	MK	2.7.1.36	3	3	0
Phosphomevalonatekinase	PVMK	2.7.4.2	8	8	0
Diphosphomevalonate decarboxylase	MVD	4.1.1.33	1	1	0
Geranyl-diphosphate synthase	GPPS	2.5.1.1	38	35	3
Farnesyl-diphosphate synthase	FPPS	2.5.1.10	19	19	0
Squalene synthase	SS	2.5.1.21	5	5	0
Squalene monooxygenase	SE	1.14.13.132	10	10	0
Cycloartenol synthase	CAS	5.4.99.8	28	15	13
Sterol 24-C-methyltransferase	SMT1	2.1.1.41	7	7	0
Methylsterol monooxygenase/Sterol-4a-methyl oxidase 2	SMO1/SMO2	1.14.13.72	7	6	1
Cycloeucalenol cycloisomerase	CEC1	5.5.1.9	1	1	0
Obtusifoliol 14-demethylase	CPY51G1	1.14.13.70	3	3	0
Delta 14-sterol reductase	FK	1.3.1.70	3	3	0
C-7,8 Sterol isomerase	HYD1	5.3.3.5	2	1	1
C-5 Sterol desaturase	STE1	1.14.21.6	12	10	2
7-Dehydro cholesterol reductase	DWF5	1.3.1.21	2	2	0
Sterol glycosyltransferases	SGT	2.4.1.173	53	42	11
Methyltransferases	MT		509	466	43