Table 4.
Antioxidant activity in methanolic extracts of adventitious root and leaves
Activity of antioxidants | Tissue | |
---|---|---|
In vitro adventitious root | In vitro leaf | |
Enzymatic antioxidants (U/mg protein) | ||
SOD | 5.66 ± 0.35 | 3.38 ± 0.25 |
Peroxidase | 3.02 ± 0.42 | 2.5 ± 0.57 |
GST | 4.0 ± 0.25 | 2.4 ± 0.09 |
Polyphenol oxidase | 3.50 ± 0.23 | 2.56 ± 0.35 |
Non-Enzymatic antioxidants (mg/g) | ||
Vitamin A | 3.54 ± 0.35 | 2.56 ± 0.25 |
Vitamin C | 3.8 ± 0.27 | 1.47 ± 0.46 |
Vitamin E | 10.2 ± 0.27 | 8.3 ± 0.57 |
Free radical scavenging activity (FRSA) | 72.5 ± 0.58% | 55 ± 0.47% |
The values of Mean ± SD of triplicates.
SOD (U/mg): amount of SOD that causes 50% reduction in the extent of NBT oxidation.
Peroxidase (U/mg): change in absorbance/min at 430 nm.
GST (U/mg protein): moles of CDNB conjucated/min/mg of sample.
Polyphenol oxidase: (U/mg protein): change in absorbance/minute at 495 nm.