Table 1.
SNP | Primers for PCR | Sequencing primer | SNP sequence | Annealing temperature |
---|---|---|---|---|
rs1800625 | F: *bio-TCTTTTTTCCCTGGGTTTAGTTGA | GAGAGAAACCTGTTTGG | AA/GCTTC | 60°C |
R: ATAGGGTTCAGGCCAGACTGTTGT |
*biotin labeling.
SNP | Primers for PCR | Sequencing primer | SNP sequence | Annealing temperature |
---|---|---|---|---|
rs1800625 | F: *bio-TCTTTTTTCCCTGGGTTTAGTTGA | GAGAGAAACCTGTTTGG | AA/GCTTC | 60°C |
R: ATAGGGTTCAGGCCAGACTGTTGT |
*biotin labeling.