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. 2014 Dec 30;15(1):423. doi: 10.1186/s12859-014-0423-x

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© Meng et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Original pre-miRNA and intercepted pre-miRNA of Solanum lycopersicum miR-166b. Mature miRNA is at 3’-end and miRNA* is at 5’-end of the selected sequence. Each base has two states, match or mismatch. Each precursor contains one loop at least. The original pre-miRNA has 201 bases with the MFE −76.92 kcal/mol and the intercepted pre-miRNA has 138 bases with the MFE −51.72 kcal/mol.