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. 2014 Dec 31;14:375. doi: 10.1186/s12870-014-0375-3

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© Segonne et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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MdPME2 cloning and validation by semi-quantitative RT-PCR. A Analysis of MDP0000222620 sequence annotated on Malus x domestica genome [22]. At1g53830 is the Arabidopsis thaliana most similar sequence. MDP0000245813 is the complete sequence identified from the 3’ RACE analysis. All 3 protein sequences are characterized by the presence of a PRO domain region (PF04043) followed by a putative processing motif [33,69]. The PME domain (PF01095) of MDP0000222620 is incomplete. B Confirmation of the cloned gene expression profiles with 2 PME specific primer sets by semi-quantitative RT-PCR for 30 cycles. M20 genomic DNA (DNAg) was used as positive control. Genes expression were studied for pair 2 (M20/M74) during complete fruit kinetic. MDP0000245813 could be amplified with clear difference between mealy (M) and non-mealy (N) genotypes, while there was no amplification with MDP0000222620, and GADPH expression remained at a relatively constant level.