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. 2015 Jan 13;2015:530371. doi: 10.1155/2015/530371

Figure 4.

Figure 4

(a) Quantitative analysis of mRNA expression by real-time PCR showed a marked increase of IL-1β mRNA expression in the brain of P6 rat pups that were kept for 24 h under hyperoxia (black bar), whereas dexmedetomidine treatment restores IL-1β upon control level (hatched grey bars) depending on the dexmedetomidine concentration. Application of dexmedetomidine under room air (grey bars) showed no significant regulation on IL-1β mRNA expression. (b) The analysis of IL-1β protein expression by western blot showed a similar expression pattern. The protein expression of IL-1β is significantly increased after 24 h of hyperoxia and a single application of 5 or 10 μg/kg dexmedetomidine could restore the IL-1β protein expression almost up to control level. The densitometric data represent the ratio of the pixel intensity of the IL-1β band to the corresponding β-actin band. Blots are representative of a series of three blots. Data are normalized to levels of rat pups exposed to normoxia (CON = 100%, white bars). Bars represent mean + SEM; n = 6 per group; *** P < 0.001 versus normoxia/control; # P < 0.05, ## P < 0.01, and ### P < 0.001 versus hyperoxia.