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P27 sequesters Cyclin E in Nodal knockdown cells. A) Western blot demonstrating co-immunoprecipitation of p27 with Cdk2 and Cyclin E. B) Two-dimensional western blotting for p27. C) Growth of control or Nodal-knockdown cells in response to siRNA-mediated loss of p27. Lower panels: Western blot analysis of p27 protein levels. All data are representative of 3 experiments.
