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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Semin Cancer Biol. 2014 Jul 26;29:40–50. doi: 10.1016/j.semcancer.2014.07.007

Figure 7.

Figure 7

Nodal regulates p27 and c-myc protein levels and post-translational modifications through ERK activation in breast cancer cells. A) A representative immunohistochemistry result shows increased expression (red brown staining) of active ERK in a Nodal positive (brown staining) breast cancer biopsy section (200× original magnification). B) Indicated cells were stimulated with rhNodal (100 ng/ml), U0126 (2μM) or EGF (50 ng/ml) for 6 hours as indicated and protein expression determined by Western blotting. C) Cells were stimulated as indicated and lysates subjected to isoelectric focusing and two-dimensional western blotting. Data are representative of two experiments. D) Targeting Nodal in breast cancer cells disables multiple pro-tumor processes, including ERK activation, downstream stabilization of c-myc, destabilization of p27 and modification of c-myc pro-tumor target gene expression.