Table 2.
In vitro permeation studies of 0.5% aqueous solution of aceclofenac from with different preservatives through excised goat, sheep, and buffalo cornea.
| Preservative |
Amount permeated (mg) (120 minutes) |
Permeation (%) (120 minutes) |
Corneal hydration (%) | Papp cm/sec ∗ 106 | Surface tension (dyne/cm) |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Goat | Sheep | Buffalo | Goat | Sheep | Buffalo | Goat | Sheep | Buffalo | Goat | Sheep | Buffalo | ||
| Control | 2.8 ± 0.002 | 1.2 ± 0.005 | 1.1 ± 0.004 | 56 | 24 | 22 | 79.1 ± 0.411 | 80.4 ± 0.001 | 79.2 ± 0.55 | 9.998 ± 0.86 | 3.36 ± 0.12 | 2.99 ± 0.36 | 69.4 |
| BAC | 4.4 ± 0.004 | 2.8 ± 0.008 | 1.7 ± 0.005 | 84 | 76 | 34 | 80.5 ± 0.145 | 78.5 ± 0.548 | 80.4 ± 0.312 | 15.01 ± 0.45 | 9.60 ± 0.03 | 4.91 ± 0.21 | 53.1 |
| MP-PP | 4.1 ± 0.003 | 3.6 ± 0.004 | 1.4 ± 0.003 | 82 | 64 | 28 | 80.2 ± 0.021 | 80.1 ± 0.245 | 79 ± 0.342 | 15.09 ± 0.40 | 8.71 ± 0.05 | 5.09 ± 0.12 | 54.7 |
| BA | 3.2 ± 0.005 | 2.4 ± 0.004 | 1.5 ± 0.009 | 63 | 50 | 30 | 80.9 ± 0.486 | 80.1 ± 0.116 | 79 ± 0.325 | 8.65 ± 0.21 | 7.01 ± 0.06 | 5.26 ± 0.32 | 64.1 |
| THM | 1.6 ± 0.009 | 1.3 ± 0.007 | 1.1 ± 0.001 | 32 | 26 | 22 | 79.2 ± 0.125 | 80.2 ± 0.658 | 80.3 ± 0.314 | 5.23 ± 0.45 | 3.48 ± 0.12 | 2.99 ± 0.68 | 60.8 |
| PMA | 1.7 ± 0.001 | 1.1 ± 0.006 | 1.0 ± 0.001 | 34 | 22 | 20 | 80.27 ± 0.129 | 80.2 ± 0.448 | 80.69 ± 0.314 | 6.454 ± 0.65 | 2.99 ± 0.15 | 2.55 ± 0.45 | 65.1 |
BAK indicates benzalkonium chloride; MP-PP, combination of methyl paraben and propylparaben; BA, benzyl alcohol; THM, thiomersal; PMA, phenyl mercuric acetate; values are mean ± SE of three corneas in each group. Statistically significant (P < 0.05) determined by one-way ANOVA followed by Dunnett's test.
Note: All the experiments were carried out in triplicates.