Table 1.
Primer Sequences Used in Quantitative Reverse Transcription Polymerase Chain Reaction to Measure Genes of Interest
| Gene | Primers | Accession Number |
|---|---|---|
| Tyrosine 3‐monooxygenase (YWAHZ; reference gene) | Fwd 5′‐CCTGGAGAAACCTGCCAAGT‐3′ | AY970970 |
| Rev 5′‐GCCAAATTCATTGTCGTACCA‐3′ | ||
| Glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH; reference gene) | Fwd 5′‐TGTAGGAGCCCGTAGGTCATCT‐3′ | DQ152956.1 |
| Rev 5′‐ TTCTCTCTGTATTCTCGAGCCATCT‐3′ | ||
| Phosphoglycerate kinase 1 (PGK1; reference gene) | Fwd 5′‐ACTCCTTGCAGCCAGTTGCT‐3′ | NM_001034299 |
| Rev 5′‐AGCACAAGCCTTCTCCACTTCT‐3′ | ||
| Beclin 1 (BECN1) | Fwd 5′‐ GAACCTCAGCCGAAGACTAAAG‐3′ | XM_004012945.1 |
| Rev 5′‐CTAAGAGGGTGTCTGTGCATTC‐3′ | ||
| BCL2/adenovirus E1B 19 kDa interacting protein 3 (BNIP3) | Fwd 5′‐GTTCCCGACTCTGCTTCTATTT‐3′ | XM_004020372.1 |
| Rev 5′‐GTCACAGTGGGAGCTCTTG‐3′ | ||
| Lysosomal‐associated membrane protein 1 (LAMP1) | Fwd 5′‐CTTGAGAGCTGGCACTTAGAA‐3′ | XM_004012369.1 |
| Rev 5′‐CTCCAACAGGAAACGGAGAA ‐3′ | ||
| Microtubule‐associated protein 1 light chain 3β (MAP1LC3B) | Fwd 5′‐ACGCTCCTTGAGAGGATCTA‐3′ | NM_022818 |
| Rev 5′‐CAGCAGCATGGTTTCCTTATTT‐3′ | ||
| Hypoxia‐inducible factor 1α (HIF‐1α) | Fwd 5′‐TGAGCTTGCTCATCAGTTGCCA‐3′ | AY485676.1 |
| Rev 5′‐ACGCAAATAGCTGATGGTGAGCCT‐3′ | ||
| Hypoxia‐inducible factor 2α (HIF‐2α) | Fwd 5′‐TACAGGTTCCTCCCCGTCAC‐3′ | NM_174725.2 |
| Rev 5′‐CTTGTCAGCTGTCATTGTCGC‐3′ | ||
| Hypoxia‐inducible factor 3α (HIF‐3α) | Fwd 5′‐GTGGAGTTCCTGGGCATCAG‐3′ | EU340262.1 |
| Rev 5′‐CCCGTCAGAAGGAAGCTCAG‐3′ | ||
| Hypoxia‐inducible factor 1β (HIF‐1β) | Fwd 5′‐AGGTGTGGCAATAGCTCTGTGGAT‐3′ | NM_173993.1 |
| Rev 5′‐AGGCCTTGATATAGCCTGTGCAGT‐3′ | ||
| Vascular endothelial growth factor A (VEGFA) | Fwd 5′‐TGTAATGACGAAAGTCTGGAG‐3′ | AF071015.1 |
| Rev 5′‐TCACCGCCTCGGCTTGTCACA‐3′ | ||
| VEGF receptor 1 (Flt1) | Fwd 5′‐CCGAAGGGAAGAAGGTGGTC‐3′ | NM_001191132.2 |
| Rev 5′‐GACTGTTGTCTCGCAGGTCA‐3′ | ||
| Angiopoietin 1 (ANGPT1; Ang1) | Fwd 5′‐TGCAAATGTGCCCTCATGCT‐3′ | AY881028.1 |
| Rev 5′‐TTCCATGGTTCTGTCCCGCT‐3′ | ||
| Angiopoietin 2 (ANGPT2; Ang2) | Fwd 5′‐AGAACCAGACCGCTGTGATG‐3′ | AY881029.1 |
| Rev 5′‐TGCAGTTTGCTTATTTCACTGGT‐3′ | ||
| Tyrosine‐protein kinase receptor (TEK/Tie2) | Fwd 5′‐CAGTTTACCAGGTGGACATC‐3′ | AY288926.1 |
| Rev 5′‐ACATTTTGGAAGGCTTGGGC‐3′ | ||
| Solute carrier family 2 (facilitated glucose transporter), member 1 (SLC2A1/GLUT1) | Fwd 5′‐ATCGTGGCCATCTTTGGCTTTGTG‐3′ | U89029.1 |
| Rev 5′‐CTGGAAGCACATGCCCACAATGAA‐3′ | ||
| Solute carrier family 2 (facilitated glucose transporter), member 3 (SLC2A3/GLUT3) | Fwd 5′‐AGAGTATGCGGATGTCGCAG‐3′ | NM_001009770.1 |
| Rev 5′‐CACCGATAGTGGCGTAGACC‐3′ | ||
| Inducible nitric oxide synthase (iNOS) | Fwd 5′‐AAGGCAGCCTGTGAGACATT‐3′ | AF223942.1 |
| Rev 5′‐CAGATTCTGCTGCGATTTGA‐3′ | ||
| Adrenomedullin (Adm) | Fwd 5′‐GGGGTGCAAGCCTCACTATT‐3′ | NM_173888.3 |
| Rev 5′‐ CACATTCCACGCAGCAAACA‐3′ | ||
| B‐cell CLL/lymphoma 2 (Bcl2) | Fwd 5′‐GTGGAGGAGCTCTTCAGGGA‐3′ | HM630309.1 |
| Rev 5′‐GTTGACGCTCTCCACACACA‐3′ | ||
| Bcl‐2 associated protein (Bax) | Fwd 5′‐CAGGATGCATCCACCAAGAAGC‐3′ | AF163774.1 |
| Rev 5′‐TTGAAGTTGCCGTCGGAAAACATT‐3′ | ||
| Protein kinase C‐ε (PKCε) | Fwd 5′‐AGCACCCGTTCTTCAAGGAG‐3′ | XM_004005978.1 |
| Rev 5′‐TGCTTGCAGCATCACCAAAC‐3′ | ||
| Egl nine homolog 2 (EGLN2/PHD‐1) | Fwd 5′‐ATGGTGGCATGTTACCCAGG‐3′ | NM_001102193.1 |
| Rev 5′‐AGGGGCTCAATGTTGGCTAC‐3′ | ||
| Egl nine homolog 1 (EGLN1/PHD2) | Fwd 5′‐TGGAGATGGAAGATGTGTGA‐3′ | NM_001206046.2 |
| Rev 5′‐TTGGGTTCAATGTCAGCAAA‐3′ | ||
| Egl nine homolog 3 (EGLN3/PHD3) | Fwd 5′‐TGCTACCCAGGAAATGGAACAGGT‐3′ | NM_001101164.1 |
| Rev 5′‐GCTTGGCATCCCAGTTCTTGTTCA‐3′ |