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. 2014 Aug 21;3(4):e001015. doi: 10.1161/JAHA.114.001015

Figure 1.

Figure 1.

Oligomerized amylin incorporates within cardiac myocytes in prediabetic humans. A and B, Western blot analysis with an antiamylin antibody on left ventricular myocyte lysates from human hearts demonstrates the presence of amylin trimers (12 kDa), tetramers (16 kDa), and octamers (32 kDa) attached to the sarcolemma or inside the myocytes (representative of 4 experiments). Nonfailing hearts (L‐NF) and failing hearts (L‐HF) from nonobese patients served as negative controls. C, Western blot experiments with antiamylin antibody on HIP myocyte lysates performed with and without DTT and SDS in the sample and running buffers. D and E, Western blot analysis with an antiamylin antibody showing the presence of amylin aggregates (trimers and tetramers) in both left ventricle protein homogenates (h) and myocyte lysates (m) from the same patient from O‐HF (C) and O‐NF (D) groups. *P<0.05 vs L‐NF. #P<0.05 vs O‐NF. DTT indicates dithiothreitol; HIP, human amylin in the pancreas; O‐HF, obese patients with heart failure; O‐NF, obese patients with nonfailing hearts; SDS, sodium dodecyl sulfate.