Figure 11.

A, Hematoxylin‐eosin–stained mesenteric white adipose tissue (mWAT) in different groups (scale bar=100 μm) and (B) quantification of adipocyte size of diameter measurements (average of 3 quantifying analyses was used per animal) in mWAT; n=3 to 5. C, Hematoxylin‐eosin–stained epididymal WAT (eWAT) in different groups (scale bar=100 μm) and (D) quantification of adipocyte diameters in eWAT; n=3 to 6. E through J, Gene expression analysis by qPCR in eWAT of WT and KD mice fed an RD or HFD; n=6 to 7. The z‐score method followed by the d'Agostino–Pearson omnibus test was used to test normality of data sets, after which the 2‐way ANOVA followed by the Bonferonni posttest were used, except in E, where the Kruskal–Wallis followed by Dunn's posttest were used as it did not pass normality test. ‡P<0.05 vs WT; θP<0.05 vs RD. HFD indicates high‐fat diet; KD, knockdown; qPCR, quantitative polymerase chain reaction; RD, regular diet; WT, wild‐type.