Table 1.
Primer Sequences Used in Quantitative RT‐PCR
| Gene | Forward Sequence (5′ to 3′) | Reverse Sequence (5′ to 3′) |
|---|---|---|
| Angplt2 | GATCCAGAGTGACCAGAATC | TCTCAGGCTTCACCAGGTAG |
| Angptl2 V76 | CTTGCAAAATGGCGTTACTTAAGC | CCAATAAACCCTCTTGCAGTTGC |
| TNFα | TGATCCGCGACGTGGAACTGG | CGACGTGGGCTACAGGCTTGTCA |
| IL‐6 | CCATAGCTACCTGGAGTACATGA | GTCCTTAGCCACTCCTTCTGTGA |
| TGFβ | ATTCCTGGCGTTACCTTGG | CCTGTATTCCGTCTCCTTGG |
| LPL | GGCTCTGCCTGAGTTGTAGAA | TCACTCGGATCCTCTCGATGA |
| Adiponectin | GTCAGTGGATCTGACGACACCAA | ATGCCTGCCATCCAACCTG |
| Leptin | CAGGATCAATGACATTTCACACAC | CTGGTCCATCTTGGACAAACTC |
| Cyclophilin A | CCGATGACGAGCCCTTGG | GCCGCCAGTGCCATTATG |
| HSL | GGCACAGACCTCTAAATCCC | CCGCTCTCCAGTTGAACC |
| SREBP1c | GAACAGACACTGGCCGAGATG | GAGGCCAGAGAAGCAGAAGAGAAG |
| SREBP2 | GTTCTGGAGACCATGGAG | AAACAAATCAGGGAACTCTC |
| Angptl3 | AGCACCAAGAACTACTCCCC | ATAAACGGCAGAGCAGTCGG |
| Angptl4 | TCCGTGGGGACCTTAACTGT | GTAGCGGCCCTTCCATGTTT |
| Citrate synthase | GCCAGTGCTTCTTCCACGAAT | CATGCCACCGTACATCATGTC |
| Cyp7a1 | AACGATACACTCTCCACCTTTG | CTGCTTTCATTGCTTCAGGG |
| PPARα | CTATTCGGCTGAAGCTGGTGTA | CAGGTCGTGTTCACAGGTAAGA |
| HMG‐CoA reductase | AGTACATTCTGGGTATTGCTGG | ACTCGCTCTAGAAAGGTCAATC |
| CD36 | GGCCAAGCTATTGCGACATGA | CAGATCCGAACACAGCGTAGA |
| FXR | TGGAGAACTCAAAATGACTCAGG | CTTTTGTAGCACATCAAGCAGG |
| Sirtuin‐1 | ATCCAGCTCAGGTGGAGGAAT | TTGACCGATGGACTCCTCACT |
| PPARγ | CCTGAAGCTCCAAGAATACC | GGTTCTTCATGAGGCCTGTT |
FXR indicates farnesoid X receptor; HMG‐CoA, 3‐hydroxy‐3‐methylglutaryl coenzyme‐A; HSL, hormone‐sensitive lipase; LPL, lipoprotein lipase; PPAR, peroxisome proliferator‐activated receptor; SREBP, sterol regulatory element binding protein; TGF, transforming growth factor; TNF, tumor necrosis factor; IL, interleukin.