Table 5.
Efficacy (Emax) and Sensitivity (EC50) to Acetylcholine in Femoral Arteries Isolated From 6‐Month‐Old WT and Angptl2 KD Male Mice Fed a Regular (RD) or High‐Fat Diet (HFD)
| WT+RD | WT+HFD | KD+RD | KD+HFD | |||||
|---|---|---|---|---|---|---|---|---|
| Emax (%) | EC50 | Emax (%) | EC50 | Emax (%) | EC50 | Emax (%) | EC50 | |
| Control | 98±1 (10) | 6.7±0.1 (10) | 98±1 (9) | 6.7±0.1 (9) | 97±1 (13) | 7.0±0.1 (13)‡ | 96±1 (10) | 6.9±0.1 (10) |
| +LNNA | 78±6 (9)§ | 5.9±0.1 (9)§ | 88±4 (7) | 6.3±0.1 (7) | 80±5 (12)§ | 6.2±0.1 (12)§ | 87±5 (9) | 6.2±0.1 (9)§ |
| +Indo | 92±6 (10) | 6.8±0.2 (10) | 96±2 (8) | 6.8±0.1 (8) | 97±1 (13) | 6.9±0.1 (13) | 96±1 (10) | 7.0±0.1 (10) |
| +LNNA+Indo | 74±7 (10)§ | 5.9±0.1 (10)§ | 81±7 (8) | 6.2±0.1 (8)§ | 65±7 (13)§ | 5.9±0.2 (13)§ | 74±6 (9) | 6.2±0.1 (9)§ |
Vessels were preconstricted to 30% to 50% of maximal diameter with phenylephrine (1 to 3 μmol/L). Emax are expressed as the percentage of the maximal diameter. Data presented as mean±SEM of (n) mice. The Kruskal–Wallis test followed by the Dunn's posttest were used. KD indicates knockdown; WT, wild‐type; LNNA, Nω‐nitro‐l‐arginine; Indo, indomethacin.
‡P<0.05 vs WT+RD; §P<0.05 vs control (within respective groups).