Table 1.
Sequences of specific primers and the profile of PCR used to amplify the DNA fragment carrying FokI, ApaI, and TaqI SNPs [28, 29].
| FokI polymorphism (rs2228570) | ApaI and TaqI polymorphisms (rs7975232 and rs731236) | |
|---|---|---|
| Primers | 5′-AGC TGG CCC TGG CAC TGA CTC TGC TCT-3′ | 5′-CAG AGC ATG GAC AGG GAG CAA G-3′ |
| 5′-ATG GAA ACA CCT TGC TTC TTC TCC CTC-3′ | 5′-GCA ACT CCT CAT GGC TGA GGT CTC A-3′ | |
|
| ||
| PCR conditions (40 cycles) | 30 sec at 95°C | 30 sec at 94°C |
| 30 sec at 70°C | 1 min at 70°C | |
| 30 sec at 72°C | 1 min at 72°C | |
|
| ||
| Length of PCR product (bp) | 265 | 740 |