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. Author manuscript; available in PMC: 2015 Jan 29.
Published in final edited form as: J Cell Physiol. 2014 Oct;229(10):1359–1368. doi: 10.1002/jcp.24569

Figure 4. PED expression is affected by CMA.

Figure 4

(A) CMA activation. PED protein level analyzed in A549 cells by western blot upon A549 serum deprivation for 24 and 48 hr or (B) in A549 cells transfected for 24 hr with LAMP-2A cDNA. (C) CMA inhibition. PED protein level analyzed by western blot upon A549 treatment with different doses of cloroquine or (D) in A549 transfected for 24 hr with ShRNA-LAMP-2A or (E) with siRNA Hsc70 for 48hr. (F) A549 transfected for 24 hr with PED-Myc cDNA and then serum deprived or treated with 10μM of chloroquine for 24 hrs or 15mM NH4CL for 4 hrs. PED Myc levels were analyzed by Western blot.