Table 3.

Results of bottleneck tests using bottleneck and M-ratio using 78% single-step mutations

			bottleneck			M-ratio P
Population	Year	N	TPM	Mode shift	M-ratio	θ = 0.05	θ = 0.1	θ = 0.3
N101	1996–1998	34	0.179	No	0.839	0.245	0.282	0.386
	2000–2001	23	0.082	No	0.809	0.145	0.171	0.263
	2002*	22	0.102	No	0.792	0.111	0.130	0.202
	2003*	26	0.326	No	0.810	0.156	0.178	0.259
	2004–2005*	23	0.002	Yes	0.690	0.011	0.012	0.021
	2006–2008	21	0.007	No	0.708	0.014	0.021	0.033
	2009–2012	31	0.367	No	0.666	0.005	0.006	0.011
S101	1996–2001†	30	0.125	No	0.894	0.507	0.545	0.671
	2006–2012†	26	0.064	No	0.788	0.105	0.115	0.183
	2008–2012	85	0.150	No	0.806	0.1500	0.162	0.249
E405	2010–2011	48	0.177	No	0.799	0.165	0.193	0.284

TPM, two-phase model.

Values in bold are significant (P ≤ 0.05) indicators of a genetic bottleneck. Varying θ values correspond with multiple prebottleneck effective population size estimates where θ = 0.05 (N_e = 25), θ = 0.1 (N_e = 50), and θ = 0.3 (N_e = 150).

^*Years during which the mange epizootic occurred.

^†Malibu Creek State Park subset of S101 population only.