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. 2015 Jan 29;13(1):e1002048. doi: 10.1371/journal.pbio.1002048

Figure 5. Sir2 is not required for production or action of the longevity factor.

Figure 5

(A) RLS analysis for sir2 fob1 double mutant yeast in 2% glucose and glucose-restricted conditions indicates that the longevity benefit of CR is lost upon migration to new plate locations without Sir2. (B) Percent increases in median life span relative to RLS on 2% glucose without migration. Data were collected from three independent experiments with n = 60. **, p < 0.01; *** p < 0.001 in comparison to the 2% not moved group. (C) RLS analysis for wild-type BY4741 in 2% glucose and glucose-restricted conditions indicates that supplementation with concentrated conditioned media from sir2 fob1 mutant yeast restores longevity benefit of CR upon migration to new plate locations. (D) Percent increases in median life span relative to RLS on 2% glucose without migration. Data were collected from three independent experiments with n = 45. ***, p < 0.001 in comparison to the 2% not moved group.