Table 1. Primary antibodies characteristics.
Antibody/species | Supplier/code/lot | Immunogen | Dilution |
---|---|---|---|
Rabbit polyclonal antinotch homolog 1, translocation-associated (Notch1) | Lifespan biosciences LS-C16928 Lot 6113061 | Synthetic peptide CQHSYSSPVDNTPSHQ N-terminal added cysteine Human notch1 intracell. domain (aa 2488–2502) | 1:300 |
Rabbit polyclonal antineurod (NeuroD1) | Lifespan biosciences LS-C9245 Lot 8030526 | Synthetic peptide DDDQKPKRRGPKKKKM Conjugated to malemide-activated KLH | 1:100 |
Human NeuroD1 (aa 76–91) | |||
Mouse monoclonal antineuron specific ßeta III tubulin [TUJ-1] | Abcam ab14545 Lot 543886 | Rat brain microtubules (whole Protein) | 1:500 |